{"id":12319,"date":"2023-02-21T11:35:08","date_gmt":"2023-02-21T03:35:08","guid":{"rendered":"https:\/\/sjinml.nus.edu.sg\/?page_id=12319"},"modified":"2023-02-24T14:42:58","modified_gmt":"2023-02-24T06:42:58","slug":"laboratory-equipment-lsm900","status":"publish","type":"page","link":"https:\/\/sjinml.nus.edu.sg\/facilities-equipment-landing\/laboratory-equipment-lsm900\/","title":{"rendered":"ZEISS Laser Confocal Microscope (LSM 900)"},"content":{"rendered":"\n<h2>\n\t\tLABORATORY EQUIPMENT\n\t<\/h2>\n<h1>\n\t\tLaser Confocal Microscope \n\t<\/h1>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sjinml.nus.edu.sg\/wp-content\/uploads\/2019\/10\/icon-wave.png\" alt=\"icon-wave\" itemprop=\"image\" height=\"6\" width=\"47\" title=\"icon-wave\" onerror=\"this.style.display='none'\" \/>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sjinml.nus.edu.sg\/wp-content\/uploads\/2023\/02\/Confocal-scaled.jpg\" alt=\"Confocal\" itemprop=\"image\" height=\"1920\" width=\"2560\" title=\"Confocal\" onerror=\"this.style.display='none'\" \/>\n\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sjinml.nus.edu.sg\/wp-content\/uploads\/2023\/02\/Confocal-3-scaled.jpg\" alt=\"Confocal 3\" itemprop=\"image\" height=\"2560\" width=\"1920\" title=\"Confocal 3\" onerror=\"this.style.display='none'\" \/>\n\t<p>Confocal Laser Scanning Microscopy (CLSM) is an optical imaging technique that is comparable in quality to scanning electron microscopy (SEM) at the same magnifications, with the addition of a spatial pinhole to focus a laser beam on a specific position on the sample. As only one position in the sample is illuminated at any one time, more time is required to scan across the entire sample, thus creating better contrast. The confocal microscope is also equipped with a TFT touchscreen monitor and motorized light control which allows z-stack scanning with a 10nm increment, thereafter going through processing to create a 3D image. The Time Series module allows users to compile images of specimen captured over time. Four diode laser of laser class 3b are available: 405nm, 488nm, 561nm and 640nm. Offline workstation installed with “ZEN 3.0 (blue edition)” available for user to post process, edit and analyse images.<\/p>\n<p>The laser source of this system is completed enclosed by an opaque barrier. Thus, the user of this system is exempted from obtaining the N3 license.<\/p>\n<p><strong>What kind of research is applicable \/ suitable for this equipment?<\/strong><\/p>\n<ul>\n<li>Users who wish to obtain high quality images or structures of specimens in their natural 3D state<\/li>\n<li>Users who wish to observe and reconstruct in detail the habitus and dissected appendages, whole specimens and any dissected parts<\/li>\n<li>For long-term digital records purposes. Photos taken by CLSM reduce the need of acquiring type specimens from museums for comparisons, which often damage type material in the midst of transfers and lab activity.<\/li>\n<\/ul>\n<p><strong>Any chargeable costs for equipment usage?<\/strong><\/p>\n<p>Yes, please approach Lab Manager or search the handbook (Section A6 – Schedule of rates for specialist facilities) for more details.<\/p>\n<p><strong>Technical specifications<\/strong><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"132\"><strong>Microscope Stand<\/strong>\n<strong>\u00a0<\/strong><\/td>\n<td width=\"469\">Motorized Z-drive (smallest increment 10 nm)\n<p>TFT touchscreen monitor with flat fine drive knob and fine drive disk (with scale and changeable)<\/p>\n<p>Motorized 6-position objective nosepiece for brightfield, darkfield, ACR and DIC<\/p>\n<p>Motorized transmitted-light illumination, motorized light control<\/p>\nBinocular phototube (100:0\/0:100) 15\u00b0\/field of view 25 mm showing upright image, 2X eyepiece for eyeglass wearers PL 10x\/field of view 25 mm<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Transmitted light illumination<\/strong><\/td>\n<td width=\"469\">Transmitted light with DIC set for LSM\nLSM ESID (LED light source and transmitted detector)<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Reflected light illumination<\/strong>\n<strong>\u00a0<\/strong><\/td>\n<td width=\"469\">Reflected-light FL BD set manual for Imager with LSM\n<p>Solid-state light source Colibri 5, type RGB-UV<\/p>\n<p>Filter set 90 HE LED for DAPI, FITC, TRITC and Cy5<\/p>\n<p>Reflector module FL EC P&C<\/p>\nReflector module bright field ACR P&C for reflected light<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Scanning Stage<\/strong>\n<strong>\u00a0<\/strong><\/td>\n<td width=\"469\">130 x 85 STEP with stepper motor (1 mm spindle pitch), including stepper controller SMC 2009, Joystick XY and mounting frame for slide 76×26 with frame size 160×116\n<p>Travel range 130 mm x 85 mm<\/p>\n<p>Speed 25 mm\/s<\/p>\n<p>Resolution: 0.1 \u00b5m<\/p>\n<p>Reproducibility: < 1 \u00b5m<\/p>\nAbsolute accuracy: +\/- 5 \u00b5m<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Objectives<\/strong>\n<strong>\u00a0<\/strong><\/td>\n<td width=\"469\">Objective EC Plan-Neofluar 1.25x\/0.03\n<p>Objective Plan-Apochromat 10x\/0.45 M27<\/p>\n<p>Objective LD Plan-Neofluar 20x\/0.4 Corr M27<\/p>\n<p>Objective LD Plan-Neofluar 40x\/0.6 Corr M27<\/p>\n<p>Objective Plan-Apochromat 63x\/1.4 Oil DIC M27<\/p>\n<p>Objective Plan-Apochromat 100x\/1.4 Oil DIC M27<\/p>\n<p>DIC slider Plan-Apo 10x\/0.45 II<\/p>\n<p>DIC slider LD PN 20x\/0.40 II<\/p>\n<p>DIC slider LD PN 40x\/0.60 II<\/p>\n<p>DIC slider PA 63x\/1.40 III<\/p>\nDIC slider Plan-Apochromat 100x\/1.4 III<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>LSM800 Scan head<\/strong><\/td>\n<td width=\"469\">LSM 900 Laser module LM URGB (D) with fiber-coupled, pigtailed and collimated lasers\n<p>Diode laser 405 nm, 5 mW, laser class 3B<\/p>\n<p>Diode laser 488 nm, 10 mW, laser class 3B<\/p>\n<p>Diode laser (SHG) 561 nm, 10 mW, laser class 3B<\/p>\n<p>Diode laser 640 nm, 5 mW, laser class 3B<\/p>\n<p>Directly modulated, dynamic range 10.000:1 effective<\/p>\n<p>LSM 900 MA-PMT detection module<\/p>\n2-channel deflecting optics<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>OEM computer system and software<\/strong><\/td>\n<td width=\"469\">Slim SuperMulti DVDRW\n<p>Real time controller imaging systems<\/p>\n<p>ZEN 3.0 system hardware license key<\/p>\n<p>ZEN module tiles & positions hardware license key<\/p>\nZEN module deconvolution hardware license key<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Offline workstation<\/strong><\/td>\n<td width=\"469\">ZEN desk hardware license key<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Accessories<\/strong><\/td>\n<td width=\"469\">Crossline micrometer 10:100, d = 26 mm\nUniversal mounting frame for petri dish and slides<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Dark incubation box<\/strong><\/td>\n<td width=\"469\">Incubator XL S1 for Axio Imager Z1 LSM (D)\nDocking station for TFT-display<\/td>\n<\/tr>\n<tr>\n<td width=\"132\"><strong>Laser safety accessories<\/strong>\n<strong>\u00a0<\/strong><\/td>\n<td width=\"469\">Light shielding cover\n<p>Laser safety lock modification (by SCS)<\/p>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p> <\/p>\n\n","protected":false},"excerpt":{"rendered":"<p>LABORATORY EQUIPMENT Laser Confocal Microscope Confocal Laser Scanning Microscopy (CLSM) is an optical imaging technique that is comparable in quality to scanning electron microscopy (SEM) at the same magnifications, with 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