{"id":12421,"date":"2023-02-21T15:42:29","date_gmt":"2023-02-21T07:42:29","guid":{"rendered":"https:\/\/sjinml.nus.edu.sg\/?page_id=12421"},"modified":"2023-02-24T14:12:26","modified_gmt":"2023-02-24T06:12:26","slug":"laboratory-equipment-pcrsystems","status":"publish","type":"page","link":"https:\/\/sjinml.nus.edu.sg\/facilities-equipment-landing\/laboratory-equipment-pcrsystems\/","title":{"rendered":"PCR Systems"},"content":{"rendered":"\r\n

LABORATORY EQUIPMENT<\/h2>\r\n

pcr systems<\/h1>\r\n

\"icon-wave\" \"\" \"\"<\/p>\r\n

Available models:<\/p>\r\n

    \r\n
  1. QuantStudio 5 0.2ml block (qPCR)<\/u><\/strong><\/li>\r\n
  2. Blue-Ray BioTech TurboCycler TCST-9622<\/u><\/strong><\/li>\r\n
  3. ProFlex PCR System<\/u><\/strong><\/li>\r\n
  4. Biorad T1000 ThermoCycler <\/u><\/strong><\/li>\r\n<\/ol>\r\n

    The PCR and qPCR systems allow researchers to amplify targeted DNA sequences in their target organism genome. With the addition of primers and Taq polymerase, the machines use the process of denaturation, annealing and extension to amplify the target genome sequence.<\/p>\r\n

    The qPCR machine, QuantStudio 5, also contains a fluorometer, which measures the fluorescent dyes that are added to samples. Using these readings, researchers can quantify the amount of target DNA as well as observe the amplification process.<\/p>\r\n

    What kind of research is applicable \/ suitable for this equipment?<\/strong><\/p>\r\n

      \r\n
    • Researchers who monitor the real time amplification of their target DNA (qPCR)<\/li>\r\n
    • Useful for researchers that are performing molecular work, can be used for purposes such as amplifying and identifying specific gene\/ segment of DNA which allow for:
      1. confirming identify of specific species of organisms
      2. identification and detection of pathogens.<\/li>\r\n<\/ul>\r\n

      Any chargeable costs for equipment usage?<\/strong><\/p>\r\n

      No<\/p>\r\n

      Technical specifications<\/strong><\/p>\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n
      Systems<\/strong><\/td>\r\nPCR<\/strong><\/td>\r\nqPCR<\/strong><\/td>\r\n<\/tr>\r\n
      Adapters available<\/strong><\/td>\r\n3 x 32 well (0.2ml PCR tubes) 1x 96 well (0.2ml PCR tubes)<\/td>\r\n96-well plate<\/td>\r\n<\/tr>\r\n
      Sample ramp rate<\/strong><\/td>\r\n\u00b1 4.4 \u00b0C\/sec<\/td>\r\n6.5\u00b0C\/sec<\/td>\r\n<\/tr>\r\n
      Temperature accuracy<\/strong><\/td>\r\n\u00b10.25\u00b0C (35\u00b0C to 99.9\u00b0C)<\/td>\r\n\u00b10.25\u00b0C (35\u00b0C to 99.9\u00b0C)<\/td>\r\n<\/tr>\r\n
      Temperature Range<\/strong><\/td>\r\n0 to 100.0 \u00b0C<\/td>\r\n0 to 100.0 \u00b0C<\/td>\r\n<\/tr>\r\n
      Temperature uniformity<\/strong><\/td>\r\n<0.5 \u00b0C (20 sec after reaching 95 \u00b0C)<\/td>\r\n0.4\u00b0C<\/td>\r\n<\/tr>\r\n
      Reaction volume<\/strong><\/td>\r\n10-80 \u00b5l<\/td>\r\n\u00a0<\/td>\r\n<\/tr>\r\n
      Sensitivity (resolution)<\/strong><\/td>\r\n\u00a0<\/td>\r\n1.5-fold difference \u00a0<\/td>\r\n<\/tr>\r\n
      Sensitivity (# of copies)<\/strong><\/td>\r\n\u00a0<\/td>\r\n1 copy<\/td>\r\n<\/tr>\r\n
      Filters \/ colours<\/strong><\/td>\r\n\u00a0<\/td>\r\n6 filters decoupled for up to 21 filter combinations<\/td>\r\n<\/tr>\r\n
      Excitation range<\/strong><\/td>\r\n\u00a0<\/td>\r\n96 wells: 450 – 680nm<\/td>\r\n<\/tr>\r\n
      Detection range<\/strong><\/td>\r\n\u00a0<\/td>\r\n96 wells: 500 – 730nm<\/td>\r\n<\/tr>\r\n
      Data acquisition<\/strong><\/td>\r\n\u00a0<\/td>\r\nWhole-plate imaging<\/td>\r\n<\/tr>\r\n
      Run time<\/strong><\/td>\r\n\u00a0<\/td>\r\nLess than 30 minutes<\/td>\r\n<\/tr>\r\n
      Multiplexing<\/strong><\/td>\r\n\u00a0<\/td>\r\nUp to 6 targets<\/td>\r\n<\/tr>\r\n
      Reagents<\/strong><\/td>\r\n\u00a0<\/td>\r\nSYBRTM Green dye TaqMan probes etc.<\/td>\r\n<\/tr>\r\n
      Key applications<\/strong><\/td>\r\n\u00a0<\/td>\r\nGene expression profiling\r\n

      Copy number analysis<\/p>\r\nMultiplexing, etc.<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n","protected":false},"excerpt":{"rendered":"

      LABORATORY EQUIPMENT pcr systems Available models: QuantStudio 5 0.2ml block (qPCR) Blue-Ray BioTech TurboCycler TCST-9622 ProFlex PCR System Biorad T1000 ThermoCycler The PCR and qPCR systems allow researchers to amplify […]<\/p>\n","protected":false},"author":595,"featured_media":0,"parent":2653,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-12421","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/12421"}],"collection":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/users\/595"}],"replies":[{"embeddable":true,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/comments?post=12421"}],"version-history":[{"count":3,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/12421\/revisions"}],"predecessor-version":[{"id":12745,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/12421\/revisions\/12745"}],"up":[{"embeddable":true,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/2653"}],"wp:attachment":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/media?parent=12421"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}