{"id":12429,"date":"2023-02-21T15:41:47","date_gmt":"2023-02-21T07:41:47","guid":{"rendered":"https:\/\/sjinml.nus.edu.sg\/?page_id=12429"},"modified":"2023-02-24T14:18:00","modified_gmt":"2023-02-24T06:18:00","slug":"laboratory-equipment-qubit3fluorometer","status":"publish","type":"page","link":"https:\/\/sjinml.nus.edu.sg\/facilities-equipment-landing\/laboratory-equipment-qubit3fluorometer\/","title":{"rendered":"Qubit 3 Fluorometer"},"content":{"rendered":"\r\n<h2>LABORATORY EQUIPMENT<\/h2>\r\n<h1>Qubit 3 Fluorometer<\/h1>\r\n<p><img loading=\"lazy\" decoding=\"async\" title=\"icon-wave\" src=\"https:\/\/sjinml.nus.edu.sg\/wp-content\/uploads\/2019\/10\/icon-wave.png\" alt=\"icon-wave\" width=\"47\" height=\"6\" \/><\/p>\r\n<p>The Qubit 3 fluorometer help researchers quantify the amount of\u00a0DNA,RNA\u00a0or protein that their samples contain, allowing them to determine if they have sufficient quantity of desired product. It is a useful check before researchers proceed with next generation sequencing (NGS), PCR or western blotting.\u00a0\u00a0<\/p>\r\n<ul>\r\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"3\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\">Can detect low concentrations of nucleic acids (10pg\/ml)\u00a0<\/li>\r\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"3\" aria-setsize=\"-1\" data-aria-posinset=\"2\" data-aria-level=\"1\">Able to target specific nucleic\u00a0acids\u00a0<\/li>\r\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"3\" aria-setsize=\"-1\" data-aria-posinset=\"3\" data-aria-level=\"1\">Requires purchase of regents\u00a0<\/li>\r\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"3\" aria-setsize=\"-1\" data-aria-posinset=\"4\" data-aria-level=\"1\">Requires\u00a0dilution\u00a0<\/li>\r\n<\/ul>\r\n<p><b>What kind of research is applicable \/ suitable for this equipment?<\/b>\u00a0<\/p>\r\n<ul>\r\n<li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"1\" aria-setsize=\"-1\" data-aria-posinset=\"1\" data-aria-level=\"1\">Those who require accurate readings of their DNA, RNA sample concentrations.\u00a0<\/li>\r\n<\/ul>\r\n<p><b>Any chargeable costs for equipment usage?<\/b>\u00a0<\/p>\r\n<p>No\u00a0<\/p>\r\n<p><strong>Technical specifications<\/strong><\/p>\r\n<table>\r\n<tbody>\r\n<tr>\r\n<td width=\"160\">Dynamic range:<\/td>\r\n<td width=\"440\">5 orders of magnitude<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Processing time:<\/td>\r\n<td width=\"440\">\u2264 5 seconds\/sample<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Light sources:<\/td>\r\n<td width=\"440\">Blue LED (max ~470 nm) Red LED (max ~635 nm)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Excitation filters:<\/td>\r\n<td width=\"440\">Blue 430-495 nm Red 600-645 nm<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Emission filters:<\/td>\r\n<td width=\"440\">Green 510-580 nm Red 665-720 nm<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Detectors:<\/td>\r\n<td width=\"440\">Photodiodes; measurement capability from 300-1000 nm<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Calibration type:<\/td>\r\n<td width=\"440\">2- or 3-point standard<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Tube type:<\/td>\r\n<td width=\"440\">0.5-mL Real Time PCR (polypropylene) tubes<\/td>\r\n<\/tr>\r\n<tr>\r\n<td width=\"160\">Warm-up time:<\/td>\r\n<td width=\"440\">&lt; 35 seconds<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n","protected":false},"excerpt":{"rendered":"<p>LABORATORY EQUIPMENT Qubit 3 Fluorometer The Qubit 3 fluorometer help researchers quantify the amount of\u00a0DNA,RNA\u00a0or protein that their samples contain, allowing them to determine if they have sufficient quantity of [&hellip;]<\/p>\n","protected":false},"author":672,"featured_media":0,"parent":2653,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-12429","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/12429"}],"collection":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/users\/672"}],"replies":[{"embeddable":true,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/comments?post=12429"}],"version-history":[{"count":5,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/12429\/revisions"}],"predecessor-version":[{"id":12746,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/12429\/revisions\/12746"}],"up":[{"embeddable":true,"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/pages\/2653"}],"wp:attachment":[{"href":"https:\/\/sjinml.nus.edu.sg\/wp-json\/wp\/v2\/media?parent=12429"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}