Histology is the study of biological tissues under a microscope. Producing the histological slides requires a series of processing through the use of three instruments – Thermo Scientific Shandon Citadel 2000 Tissue Processor, Leica RM2135 Rotary Microtome and the Shandon Histo-Center II. The Tissue Processor is an automated machine that assists in the fixing and dehydration of specimen. The Histo-Center II does the paraffin wax embedding of specimen and the Rotary Microtome does the slicing to produce the slides.
What kind of research is applicable / suitable for this equipment?
- Observe the structure and cells of their soft-bodied specimens in detail, under the microscope.
Any chargeable costs for equipment usage?
Yes, please approach Lab Manager or search the handbook (Section A6 – Schedule of rates for specialist facilities) for more details. Costs of chemicals are included in equipment usage charges.
Technical specifications
Chemicals required | Histo-clear, Absolute Ethanol, Paraffin wax, Haematoxylin, Eosin Y solution, Canada Balsam, DPX Mountant |
Microscope slides dimensions | 25mm by 75mm |
Histological sections thickness | 5-60 μm |