LABORATORY EQUIPMENT
microplate reader
The BioTek Synergy H1 Microplate Reader allows researchers to test multiple samples at once, with the reactions typically conducted in 96 well plates. Similar to spectrophotometers, they work on the principle of absorbance.
- 6 up to 96 well plates
- Abs wavelength 230-999nm
- Incubation of up to 45 degrees celcius
What kind of research is applicable / suitable for this equipment?
- Those who need to read the results of their elisa test. Can also read other reactions that were conducted on 96 well plates.
Any chargeable costs for equipment usage?
No.
Technical specifications
| Detection modes | UV-Vis absorbance
Fluorescence intensity Luminescence |
| Read methods | Endpoint
Kinetic Spectral scanning Well area scanning |
| Microplate types | 6- to 384-well plates |
| Temperature control | 4-Zone™ incubation to 45 °C with Condensation Control™ +0.2 °C at 37 °C |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5 Data Analysis Software |
| Abs Wavelength selection | Monochromator |
| Abs Wavelength range | 230 – 999nm
1nm increments |
| Abs Monochromator bandwidth | 4nm (230 – 285nm), 8nm (> 285nm) |
| Abs Dynamic range / Resolution | 0 – 4.0 OD / 0.0001 OD |
| Abs Monochromator wavelength accuracy / repeatability | ±2 nm / ±0.2 nm
|
| Abs OD accuracy / linearity / repeatability | <1% at 2.0 OD; <3% at 3.0 OD / <1% from 0 to 3.0 OD / <0.5% at 2.0 OD |
| Abs Reading speed | 96 wells: 11 seconds |
| Fluor. Fluorescence detector | PMT |
| Fluor. Wavelength range | 250 – 700 nm |
| Fluor. bandwidth | Fixed, 16nm |
| Fluor. Dynamic range | 7 decades |
| Fluor. Sensitivity | Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) – top; Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom |
| Luminescence Wavelength | 300 – 700 nm |
| Lumi. Dynamic range | > 6 decades |
| Lumi. Sensitivity | Monos: 20 amol ATP (flash) |
