LABORATORY EQUIPMENT

uv-vis spectrophotometer

icon-wave UV-vis Spectrophotometer 2 UV-vis Spectrophotometer

The UV-vis spectrophotometer is an analytical instrument used to study the amount or concentration of different samples based on the amount of light absorbed in the ultraviolet and visible light region. Based on Beer Lambert’s Law, the absorbance of a coloured solution is directly correlated to its concentration.

What kind of research is applicable / suitable for this equipment?

  • Extrapolating and calculating the concentration of unknown coloured samples
  • can be used in a wide variety of applications such as the quantification of nucleic acids, nutrient samples, chlorophyll count as well as measuring microalgae cultures.

Any chargeable costs for equipment usage?

Yes, please approach Lab Manager or search the handbook (Section A6 – Schedule of rates for specialist facilities) for more details.

Technical specifications

Wavelength range 185nm – 1400nm
Wavelength accuracy ±0.1nm at 656.1nm D2 peak ±0.3nm for all ranges
Wavelength repeatability ±0.05nm
Wavelength scanning speed Slew rate: 14000nm/min Scan rate: 4000 to 0.5nm/min
Resolution 0.1nm
Noise level 0.00003 Abs RMS (500nm)
Cell holders Multi-cell sample compartment: up to six 10mm square cells on sample side

Powdered sample holder: attachment to integrating sphere

Film holder: Min size – W16 x H32mm; Max size – W80 x H40 x T20 mm

Cylindrical cell holder: two cylindrical cells with optical path length of 10, 20, 50, 100mm

Supermicro cell holder: sample volume range 50 – 200uL
Software UVProbe Software

Photometric and quantitation module

Kinetics module (time course measurement)

Spectrum module

Report generator