LABORATORY EQUIPMENT
uv-vis spectrophotometer
The UV-vis spectrophotometer is an analytical instrument used to study the amount or concentration of different samples based on the amount of light absorbed in the ultraviolet and visible light region. Based on Beer Lambert’s Law, the absorbance of a coloured solution is directly correlated to its concentration.
What kind of research is applicable / suitable for this equipment?
- Extrapolating and calculating the concentration of unknown coloured samples
- can be used in a wide variety of applications such as the quantification of nucleic acids, nutrient samples, chlorophyll count as well as measuring microalgae cultures.
Any chargeable costs for equipment usage?
Yes, please approach Lab Manager or search the handbook (Section A6 – Schedule of rates for specialist facilities) for more details.
Technical specifications
| Wavelength range | 185nm – 1400nm |
| Wavelength accuracy | ±0.1nm at 656.1nm D2 peak
±0.3nm for all ranges |
| Wavelength repeatability | ±0.05nm |
| Wavelength scanning speed | Slew rate: 14000nm/min
Scan rate: 4000 to 0.5nm/min |
| Resolution | 0.1nm |
| Noise level | 0.00003 Abs RMS (500nm) |
| Cell holders | Multi-cell sample compartment: up to six 10mm square cells on sample side
Powdered sample holder: attachment to integrating sphere Film holder: Min size – W16 x H32mm; Max size – W80 x H40 x T20 mm Cylindrical cell holder: two cylindrical cells with optical path length of 10, 20, 50, 100mm Supermicro cell holder: sample volume range 50 - 200mL |
| Software | UVProbe Software
Photometric and quantitation module Kinetics module (time course measurement) Spectrum module Report generator |
